Data Storage Space NGS

[analyst]

Can anyone please help me with my inquiry.

I will have a next-generation sequencing data coming from external source. How much will be data size on disk (in Gb) for a next-generation sequencing dataset. An estimate will be fine as we want to decide on storage space to have for this.

Mouse Samples - 400
Platform - illumina
Single end

Anything else you want to ask please let me know. Also for analysis using bowtie/cufflink and other standard tool, how much additional space is advisable.

[ffinkernagel]

It very much depends on your downstream analysis.
Raw data currently runs between 0.5-1 GB of (compressed) sequence for a single illumina lane, but is still increasing.

You need at the very least that much again for the aligned data etc, and more for further processing. Not to mention temporary disk space. My 'worst' project runs around a factor of 8.5 on top of the raw data.

Just keep in mind, storage is not just raw disk space - you want redundancy, backup and checksums on your data, and it will always be cheaper to buy the same amount of storage next year.