Hi all,
I would like to introduce G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq.
More detailed information here.
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Genome Biol. 2008 Dec 16;9(12):R175.
Annotating genomes with massive-scale RNA-sequencing.
Denoeud F, Aury JM, Da Silva C, Noel B, Rogier O, Delledone M, Morgante M, Valle G, Wincker P, Scarpelli C, Jaillon O, Artiguenave F.
ABSTRACT: Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty to align short reads on exon/exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, i.e. in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
PMID: 19087247 [PubMed - as supplied by publisher]
I would like to introduce G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq.
More detailed information here.
-------------------------------------------------------------------------------------------------------------
Genome Biol. 2008 Dec 16;9(12):R175.
Annotating genomes with massive-scale RNA-sequencing.
Denoeud F, Aury JM, Da Silva C, Noel B, Rogier O, Delledone M, Morgante M, Valle G, Wincker P, Scarpelli C, Jaillon O, Artiguenave F.
ABSTRACT: Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty to align short reads on exon/exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, i.e. in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
PMID: 19087247 [PubMed - as supplied by publisher]
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