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Are you using your own barcodes or the Illumina multiplexing kit? The most recent version of NextGENe handles the demultiplexing of the data from the kits. I'm also trying to get it to work with CLC Genomics Workbench, but so far they aren't sorting right. Both of these programs cost $$ and I'm unfortunately not aware of free software that does it, although I'm sure it exists and someone else might know. Illumina software (CASAVA) will demultiplex data generated from their kits. It sorts into bins 1-24 (or 1-48?) but make sure to check what adapter/barcode is sorted into each bin because the numbers 1-24 don't correspond to adapters 1-24. I hope that helps!
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Demultiplexing Illumina reads
I did some searching and came up with very little about how to effectively demultiplex illumina data off the GAiix platform. Does anyone know of a good software package or workflow I could read through and experiment with? Sorry if this question has already been asked, I wasn't able to find much information thoughTags: None
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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