Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Fumumumu
    Junior Member
    • Jan 2011
    • 3

    #1

    Error in samtools merge; different target sequence name

    I am now making a script with bowtie and samtools, and
    troubled in merging bam files from bowtie.
    1) bowtie with every chromosome ewbt; output is sam file
    2) sam to bam (every chromosome sam file)
    3)* merge all bams
    4) bam sort
    5) bam index

    #!/bin/sh
    date=`date +"%Y%m%d_%H%M"`
    samtools merge ${date}.bam ${date}_bam_chr1.bam, ${date}_bam_chr2.bam,... ,${date}_bam_chrY.bam

    error massage was
    [bam_merge_core] different target sequence name : 'chr1' != 'chr2' in file 20110115_0050_bam_chr2.bam

    In this case, date was 20110115_0050.
    What was wrong??
  • jmrosa
    Junior Member
    • Sep 2010
    • 3

    #2
    Hi,

    I had the same problem.

    Check that chromosomes headers are in the same order in the headers of all the bam files.

    Cheers

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...