Does anyone know how bioscope decides to Hard Clip vs calling a mismatch ?
For eg :
1781_585_1567 0 validated 586867 54 34M1H * 0
0 ==================================
IIIIIIIIIIII:EI<FIIDDIIIIIII@EIIII RG:Z:20110112194907919
NH:i:1 CM:i:0 CQ:Z:<BBAB>A=B?BB4'?2+<@:+:@A@=</<%A4?>?
CS:Z:T11212021130133312020233301002211032 NM:i:0 MD:Z:34
Why is the above read are not classified as 35 bp read length with 1 mismatch?
Thanks!
For eg :
1781_585_1567 0 validated 586867 54 34M1H * 0
0 ==================================
IIIIIIIIIIII:EI<FIIDDIIIIIII@EIIII RG:Z:20110112194907919
NH:i:1 CM:i:0 CQ:Z:<BBAB>A=B?BB4'?2+<@:+:@A@=</<%A4?>?
CS:Z:T11212021130133312020233301002211032 NM:i:0 MD:Z:34
Why is the above read are not classified as 35 bp read length with 1 mismatch?
Thanks!