Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • dsidote
    Member
    • Aug 2009
    • 23

    #1

    Bioscope clip vs mismatch

    Does anyone know how bioscope decides to Hard Clip vs calling a mismatch ?

    For eg :

    1781_585_1567 0 validated 586867 54 34M1H * 0
    0 ==================================
    IIIIIIIIIIII:EI<FIIDDIIIIIII@EIIII RG:Z:20110112194907919
    NH:i:1 CM:i:0 CQ:Z:<BBAB>A=B?BB4'?2+<@:+:@A@=</<%A4?>?
    CS:Z:T11212021130133312020233301002211032 NM:i:0 MD:Z:34

    Why is the above read are not classified as 35 bp read length with 1 mismatch?

    Thanks!

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...