Hello,
I have some questions about off-target. If I have understand well off-target can rise from probes that have promiscuous hybridization and recognize more sites in the genome, but also it can rise from libraries that have too large fragments. In this latest case which is the problem if I have probes that are designed adjacent to one another? They capture only the on target and the alignment software should align only using my manifest and not the off target, or there are other problems?
In general I know that today the softwares remove the off target, so which is the problem to have it? It decreases the total coverage of the on target? And in this case in which way?
Thank you in advance!
I have some questions about off-target. If I have understand well off-target can rise from probes that have promiscuous hybridization and recognize more sites in the genome, but also it can rise from libraries that have too large fragments. In this latest case which is the problem if I have probes that are designed adjacent to one another? They capture only the on target and the alignment software should align only using my manifest and not the off target, or there are other problems?
In general I know that today the softwares remove the off target, so which is the problem to have it? It decreases the total coverage of the on target? And in this case in which way?
Thank you in advance!
