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  • dagarfield
    replied
    Maybe this will be useful?

    Leave a comment:


  • Tahir Mujtaba
    replied
    GFF to BED

    How to convert a GFF file intO BED format file?.

    Leave a comment:


  • ketan_bnf
    replied
    Originally posted by quinlana View Post
    The awesome power of awk and sed.

    Code:
    sed -e 's/chr//' file.vcf | awk '{OFS="\t"; if (!/^#/){print $1,$2-1,$2,$4"/"$5,"+"}}'
    where you replace "file.vcf" with your file. Note this uses 0-based start coordinates, which are the proper BED format. If you want to use 1-based, change "$2-1" to just "$2".
    Thanks for reply,

    if suppose i want to put "-" reverse strand instead of "+", how can i put that using vcf file?

    1 46707 46707 A/G +
    1 168576 168576 C/T +
    1 168635 168635 G/A +
    1 195648 195648 T/G -

    Leave a comment:


  • quinlana
    replied
    The awesome power of awk and sed.

    Code:
    sed -e 's/chr//' file.vcf | awk '{OFS="\t"; if (!/^#/){print $1,$2-1,$2,$4"/"$5,"+"}}'
    where you replace "file.vcf" with your file. Note this uses 0-based start coordinates, which are the proper BED format. If you want to use 1-based, change "$2-1" to just "$2".

    Leave a comment:


  • ketan_bnf
    started a topic Converter for vcf to bed format

    Converter for vcf to bed format

    Hi all,

    Is there any converter that can convert vcf format files obtained by samtools vcfutils.pl to bed format?

    Because i want predict the variant effect using varian_effect_predictor of ensemble.

    ##fileformat=VCFv4.0
    #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT GKUNU9Q04_chr1_sort.bam
    chr1 46707 . A G 7.59 . DP=2;AF1=1;CI95=0.5,1;DP4=0,0,0,2;MQ=60 PL:GT:GQ 38,6,0:1/1:49
    chr1 168576 . C T 4.61 . DP=2;AF1=1;CI95=0.5,1;DP4=0,0,0,2;MQ=60 PL:GT:GQ 34,6,0:1/1:49
    chr1 168635 . G A 17.1 . DP=3;AF1=1;CI95=0.5,1;DP4=0,0,0,3;MQ=60 PL:GT:GQ 49,9,0:1/1:63

    To

    1 46707 46707 A/G +
    1 168576 168576 C/T +
    1 168635 168635 G/A +

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