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  • Marius
    8armed
    • Dec 2010
    • 30

    #1

    Combining multiple Piccard commands with Bash

    I'm new to using bash for piping. I'm currently trying to combine (pipe) multiple Piccard commands with Bash.
    I have two executions that both work when I run them one by one in a bash script:

    **********************************************

    Command 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    done

    Command 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done

    **********************************************

    I have now tried to pipe these commands, but it no longer works. I have tried 2 ways of doing the piping:


    Option 1:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=/dev/stdout SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT | \
    java -jar $picardtools AddOrReplaceReadGroups INPUT=/dev/stdin OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Option 2:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Neither of these two options work. Can someone help me finding the bug?

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