Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Marius
    8armed
    • Dec 2010
    • 30

    #1

    Combining multiple Piccard commands with Bash

    I'm new to using bash for piping. I'm currently trying to combine (pipe) multiple Piccard commands with Bash.
    I have two executions that both work when I run them one by one in a bash script:

    **********************************************

    Command 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    done

    Command 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done

    **********************************************

    I have now tried to pipe these commands, but it no longer works. I have tried 2 ways of doing the piping:


    Option 1:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=/dev/stdout SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT | \
    java -jar $picardtools AddOrReplaceReadGroups INPUT=/dev/stdin OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Option 2:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Neither of these two options work. Can someone help me finding the bug?

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
26 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
20 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
43 views
0 reactions
Last Post SEQadmin2  
Working...