Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Marius
    8armed
    • Dec 2010
    • 30

    #1

    Combining multiple Piccard commands with Bash

    I'm new to using bash for piping. I'm currently trying to combine (pipe) multiple Piccard commands with Bash.
    I have two executions that both work when I run them one by one in a bash script:

    **********************************************

    Command 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    done

    Command 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done

    **********************************************

    I have now tried to pipe these commands, but it no longer works. I have tried 2 ways of doing the piping:


    Option 1:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=/dev/stdout SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT | \
    java -jar $picardtools AddOrReplaceReadGroups INPUT=/dev/stdin OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Option 2:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Neither of these two options work. Can someone help me finding the bug?

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM
  • SEQadmin2
    Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
    by SEQadmin2



    Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
    ...
    07-09-2026, 11:10 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 07-31-2026, 02:55 AM
0 responses
14 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-24-2026, 12:17 PM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-23-2026, 11:41 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 07-20-2026, 11:10 AM
0 responses
24 views
0 reactions
Last Post SEQadmin2  
Working...