Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Marius
    8armed
    • Dec 2010
    • 30

    #1

    Problem using BASH to pipe Piccard command

    I'm new to Bash, but would like to use it.
    I would like to pipe two Picard (GATK pipeline) commands using Bash. Each of these commands works fine when they are run as individual Bash scripts (see below). However, when I try to combine them within a single bash script, it no longer works. I really cannot find the bug though. Help is much appreciated.


    BASH Script 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outSorted='PATH/outSorted'

    for i in BamFile1 BamFile2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outSorted/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    done


    BASH Script 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    outSorted='PATH/outSorted'
    outSorted2='PATH/outSorted2'

    for i in BamFile1 BamFile2
    do
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outSorted/$i OUTPUT=$outSorted2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    ######################

    Here my attempts to pipe the two Scripts into one. But neither of these attempts worked. Can you help me find out why?

    Attempt 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outSorted='PATH/outSorted'
    outSorted2='PATH/outSorted2'

    for i in BamFile1 BamFile2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outSorted/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outSorted/$i OUTPUT=$outSorted2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Attempt 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outSorted2='PATH/outSorted2'

    for i in BamFile1 BamFile2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=/dev/stdout SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT | \
    java -jar $picardtools AddOrReplaceReadGroups INPUT=/dev/stdin OUTPUT=$outSorted2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...