Illegal division by zero at sissrs.pl line 263.

[SDBP]

Hi,
I am trying to run the SISSRS perl script for chipseq analysis but get the following error:

Processing...
Reading data file...done!
Illegal division by zero at sissrs.pl line 263.



Anyone else got this error?
Any remedies?

tx.

[biznatch]

Line 263 from SISSRS is:

$posFrac = $numPositive/$tagsSelected*100;

so $tagsSelected must be zero, so maybe it's not finding any peaks in your file.

What command/options are you using and can you post the first few lines of your bed file(s)?

[SDBP]

Solved yesterday.

Yeah.Thanks for replying.
I figured it out yesterdayand could run the script and got the output.
I had to correct the format in the bed file.

[Pravara_@bioinformatics]

related to chip-seq and bed file

i m analysing chip-seq data ....but i have .peak files also alongwith .bed files....it's working ..but i got an error same as mentioned above.

Illegal division by zero at sissrs.pl line 263.

peak file format is like

chr4 184505570 184508116 1215 86 14.268423408896 1129 0
chr19 55219872 55221447 1737 235 7.40194575291536 1502 0
chr21 35181531 35184550 1687 243 6.97093250755022 1444 0

if anyone knws the solution of this problem plz let me knw....