Hi Everyone,
The CADD data set from Washington U., lists annotation for all potential variants in the human genome, including annotation about amino acid changes. The single letter amino acid code is used. In numerous cases the code 'U' is used for the original, ie reference, amino acid. This is clearly not in the IUPAC single-letter amino acid table.
Can anyone tell me what this refers to, and why? This seems like an error, but maybe there was a reason for it. Either way, its not an IUPAC code, so really shouldn't be used.
Has anyone else had problems with this?
Joe White
Wuxi NextCODE Genomics
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM