I have gene expression data from different conditions from different studies. Instead of using the actual TPM values for Pearson Correlation coefficient (PCC) calculation, I have decided to use Fold change values from different studies to eliminate biases from different studies. My question is whether using these raw fold change values for identifying co-expressed genes is a correct way to do it or should perform quantile normalization on these fold change values before using them for PCC calculation? (Note: Distribution of fold change values in different studies is quite different)
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How to perform Pearson correlation coefficient calculations on RNA seq fold change va
Last edited by Jayesh; 12-19-2019, 10:30 AM.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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