WHAT: 2-day Technical Training in NGS Data Analysis | Whole Genome (DNA) Sequencing & RNASeq in Mumbai, India

WHEN: 6-7 March 2020

It will be an extensive 2-day training program for which participants have to bring their own laptop having Windows/Mac/Linux so the participants can use that for running the processes on their own computer systems- HANDS-ON with complete learning of Next Generation Sequencing Data Analysis. The program will be of great help to students/ researchers/ faculty/ scientists in learning the analysis of NGS DNA & RNA Data.

Event Website

PRACTICAL APPLICATION
In the workshop, we will perform 2 experiments to analyze the example DNASeq & RNASeq Data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.

SCHEDULE | DAY 1 EXPERIMENT 1
In this lesson, we will focus on the SNPs and Variant Analysis (VC) of the DNASeq data and cover:
  • Deal with the raw data for quality evaluation and filtering
  • Map reads to the reference genomes, call SNPs and predict their phenotype consequence
  • Alignment to a reference genome, SNPs and In-dels Calling of next generation DNA data


SCHEDULE | DAY 2
EXPERIMENT 2 In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
  • Upload Data
  • Convert File Format
  • Align Reads To Reference Genome
  • View The Mapped Reads In JBrowse
  • Count Reads Per Gene
  • Test For Differential Expression
  • Differential Gene Expression
  • MDS Plot
  • MA Plot
  • Volcano Plot
  • Parallel Coordinates and Heatmap
  • Investigate Differentially-Expressed Genes
  • Chord Diagram