WHAT: 2-day Technical Training in NGS Data Analysis | Whole Genome (DNA) Sequencing & RNASeq in Mumbai, India

WHEN: 6-7 March 2020

It will be an extensive 2-day training program for which participants have to bring their own laptop having Windows/Mac/Linux so the participants can use that for running the processes on their own computer systems- HANDS-ON with complete learning of Next Generation Sequencing Data Analysis. The program will be of great help to students/ researchers/ faculty/ scientists in learning the analysis of NGS DNA & RNA Data.

Event Website

In the workshop, we will perform 2 experiments to analyze the example DNASeq & RNASeq Data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.

In this lesson, we will focus on the SNPs and Variant Analysis (VC) of the DNASeq data and cover:
  • Deal with the raw data for quality evaluation and filtering
  • Map reads to the reference genomes, call SNPs and predict their phenotype consequence
  • Alignment to a reference genome, SNPs and In-dels Calling of next generation DNA data

EXPERIMENT 2 In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
  • Upload Data
  • Convert File Format
  • Align Reads To Reference Genome
  • View The Mapped Reads In JBrowse
  • Count Reads Per Gene
  • Test For Differential Expression
  • Differential Gene Expression
  • MDS Plot
  • MA Plot
  • Volcano Plot
  • Parallel Coordinates and Heatmap
  • Investigate Differentially-Expressed Genes
  • Chord Diagram