WHEN: 6-7 March 2020
It will be an extensive 2-day training program for which participants have to bring their own laptop having Windows/Mac/Linux so the participants can use that for running the processes on their own computer systems- HANDS-ON with complete learning of Next Generation Sequencing Data Analysis. The program will be of great help to students/ researchers/ faculty/ scientists in learning the analysis of NGS DNA & RNA Data.
Event Website
PRACTICAL APPLICATION
In the workshop, we will perform 2 experiments to analyze the example DNASeq & RNASeq Data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.
SCHEDULE | DAY 1 EXPERIMENT 1
In this lesson, we will focus on the SNPs and Variant Analysis (VC) of the DNASeq data and cover:
- Deal with the raw data for quality evaluation and filtering
- Map reads to the reference genomes, call SNPs and predict their phenotype consequence
- Alignment to a reference genome, SNPs and In-dels Calling of next generation DNA data
SCHEDULE | DAY 2
EXPERIMENT 2 In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
- Upload Data
- Convert File Format
- Align Reads To Reference Genome
- View The Mapped Reads In JBrowse
- Count Reads Per Gene
- Test For Differential Expression
- Differential Gene Expression
- MDS Plot
- MA Plot
- Volcano Plot
- Parallel Coordinates and Heatmap
- Investigate Differentially-Expressed Genes
- Chord Diagram