Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • adamhfreedman
    Junior Member
    • Oct 2010
    • 8

    #1

    dwgsim arguments not working (on mac)

    i can get dwgsim to generate the help menu, and if i stick the command line arguments after the reference genome file and outfile.fq names, it runs fine but uses the default settings, ignoring the arguments.

    the correct order, per the wiki is:

    dwgsim [options] <in.ref.fa> <out.bwa.read1.fq> <out.bwa.read2.fq> <out.bfast.fq>

    but when i do this, .e.g:
    dwgsim -e 0.01 -E 0.01 -d 500 -s 50 -N 100000 -1 100 -2 100 -r 0.0010 -R 0.10 -X 0.3 -n 0 /Volumes/Storage/CanidGenomes_Project/CanFam3/canfam3.fa out.bwa.read1.fq out.bwa.read2.fq out.bfast.fq

    i get the following error:

    [dwgsim_core] fail to open file '0.01'. Abort!

    basically, dwgsim is trying to read the first argument as the reference file name.

    is there some sort of mac specific argument parsing problem here, and if so, a solution?
    Adam H. Freedman
    Dept. of OEB, Harvard University
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    Please run the version from the latest git and post your results. The options have changed, so please adjust accordingly.

    Comment

    • adamhfreedman
      Junior Member
      • Oct 2010
      • 8

      #3
      solved it, but error during make

      Thanks Nils, it works now.

      The only other thing is that there were errors during make:

      make[2]: *** No rule to make target `dwgsim_eval.pl', needed by `all-am'. Stop.
      make[1]: *** [all-recursive] Error 1
      make: *** [all] Error 2
      Adam H. Freedman
      Dept. of OEB, Harvard University

      Comment

      • nilshomer
        Nils Homer
        • Nov 2008
        • 1283

        #4
        Originally posted by adamhfreedman View Post
        Thanks Nils, it works now.

        The only other thing is that there were errors during make:

        make[2]: *** No rule to make target `dwgsim_eval.pl', needed by `all-am'. Stop.
        make[1]: *** [all-recursive] Error 1
        make: *** [all] Error 2
        Thanks for the report, fixed in a new commit.

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM
        • SEQadmin2
          New Genomics Technologies Take Aim at Long-Standing Limits
          by SEQadmin2


          Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

          We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
          ...
          09-28-2026, 10:25 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 11:13 AM
        0 responses
        16 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, Yesterday, 10:48 AM
        0 responses
        16 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-15-2026, 10:52 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-14-2026, 05:39 AM
        0 responses
        42 views
        0 reactions
        Last Post SEQadmin2  
        Working...