read group in SAM format

[yinshe]

Hi,

Maybe this question is not so technically related. In SAM format specification, in the description of "header section" about @RG, it says:

"Read group IDs may be modified when merging SAM files in order to handle collisions."

Does anybody have experience about how the IDs will be changed?

thanks for any help!

[csoong]

according to your needs. if you got to dataset being merged with different RG but are really the same you could modify it to become the same. if you got 2 dataset with same RG but actually different samples, you may modify one of them so the RG will reflect they are different.

[yinshe]

Thanks for quick reply! Now I understand, so I need to modify RG manually. The tool can not automatically change the RG.