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  • ritzriya
    Member
    • Jun 2010
    • 49

    Is a Gene mapping tool available?

    Dear All,

    I have got Genes/CDS from the RNA-Seq data. I want to map these genes to the genome of that organism. Which tool will make it possible ?

    I have used Blastn and Megablast till now. But my main problem is that, say a gene is of length 600 bp, but it matches to the genome 100% only to 200bp of its length. Then, should I consider that this gene has mapped? If yes, then how will I give the co-ordinates?

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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  • SEQadmin2
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    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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