Tophat :: CIGAR for second read in pair - SEQanswers

You are currently viewing the SEQanswers forums as a guest, which limits your access. Click here to register now, and join the discussion

X

polarise

Member

Join Date: Jan 2011

Posts: 13
- Share
- Tweet
#1

Tophat :: CIGAR for second read in pair

02-16-2011, 04:33 AM

Hi SEQers,

Is it correct to say that, when performing an alignment with paired-ended reads, that the CIGAR alignment data for the second read is lost?

Thanks.

Paul
Tags: None

Previous template Next

From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data

by SEQadmin2

Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...
- Channel: Articles
06-02-2026, 10:05 AM
Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends

by SEQadmin2

With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.

Introduction

Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
- Channel: Articles
05-22-2026, 06:42 AM

	Topics		Statistics	Last Post
	A New Method Makes Hantavirus Genome Analysis Faster and More Accessible by SEQadmin2 Started by SEQadmin2, 06-05-2026, 10:09 AM		0 responses 10 views 0 reactions	Last Post by SEQadmin2 06-05-2026, 10:09 AM
	A New Single-Cell Method Maps DNA-Protein Interactions by SEQadmin2 Started by SEQadmin2, 06-04-2026, 08:59 AM		0 responses 22 views 0 reactions	Last Post by SEQadmin2 06-04-2026, 08:59 AM
	Long-Read RNA Sequencing Uncovers a Hidden Layer of Immune Cell Regulation by SEQadmin2 Started by SEQadmin2, 06-02-2026, 12:03 PM		0 responses 28 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 12:03 PM
	DNA Methylation Study Reveals How Epigenetic Changes Pass Between Generations by SEQadmin2 Started by SEQadmin2, 06-02-2026, 11:40 AM		0 responses 22 views 0 reactions	Last Post by SEQadmin2 06-02-2026, 11:40 AM

Working...