No problem, good luck with your further analysis.
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bowtie-build error
Hi boetsie,Originally posted by boetsie View Post
SSPACE must be very useful tool for scaffolding. But when I tried to use it, the process was failed by bowtie-build step. I only have contig file contains all name with super_contig sequences without other information and there are lots of 'N' gaps between. Do I need to modify some information and get bowtie-build works? If not, what's the problem?
The reads I have are 100PE
so the library is like
lib1 ***1.fastq ***2.fastq 200 0.7 0
or I should replace 200 to 400?Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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