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  • ameyer
    Member
    • Jan 2011
    • 23

    #1

    Cufflinks error

    Hi,
    I'm having a problem getting Cufflinks to run.
    I used TopHat to align my reads but when running Cufflinks it gives the error:
    Error: sort order of reads in BAMs must be the same.
    I've tried converting the .bam file to .sam and it still gave the error so I then used the sort command given in the Cufflinks manual (sort -k 3,3 -k 4,4n accepted_hits.sam > hits.sam.sorted) but it still gave the error.
    Why is it doing this?
    Thanks
  • jtrivino
    Junior Member
    • Feb 2011
    • 4

    #2
    Hi

    I use
    samtools sort *.bam *_sort.bam
    samtools index sort.bam

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