Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • richardallenfriedman
    Junior Member
    • Nov 2020
    • 1

    #1

    Interpreation of RSEQC read_distribution.py results

    Dear Seqanswers List,

    Here is my ouput from read_distribution.py from RSEQC run from Galaxy

    Total Reads 54875279
    Total Tags 58170116
    Total Assigned Tags 54031503
    =====================================================================
    Group Total_bases Tag_count Tags/Kb
    CDS_Exons 39184887 10217883 260.76
    5'UTR_Exons 37214570 10963357 294.60
    3'UTR_Exons 58010731 4386101 75.61
    Introns 1539911688 6018229 3.91
    TSS_up_1kb 24518972 906468 36.97
    TSS_up_5kb 110212503 4956374 44.97
    TSS_up_10kb 201827137 13004223 64.43
    TES_down_1kb 27333233 59583 2.18
    TES_down_5kb 118264548 3689226 31.19
    TES_down_10kb 210002921 9441710 44.96

    My questions:
    Where does rRNA fit in this picture?
    What about other transcripts other than mRNA such as pseudogenes?

    Thanks and best wishes,
    Rich
    Richard Friedman,
    Columbia University

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
54 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...