Dear Seqanswers List,
Here is my ouput from read_distribution.py from RSEQC run from Galaxy
Total Reads 54875279
Total Tags 58170116
Total Assigned Tags 54031503
=====================================================================
Group Total_bases Tag_count Tags/Kb
CDS_Exons 39184887 10217883 260.76
5'UTR_Exons 37214570 10963357 294.60
3'UTR_Exons 58010731 4386101 75.61
Introns 1539911688 6018229 3.91
TSS_up_1kb 24518972 906468 36.97
TSS_up_5kb 110212503 4956374 44.97
TSS_up_10kb 201827137 13004223 64.43
TES_down_1kb 27333233 59583 2.18
TES_down_5kb 118264548 3689226 31.19
TES_down_10kb 210002921 9441710 44.96
My questions:
Where does rRNA fit in this picture?
What about other transcripts other than mRNA such as pseudogenes?
Thanks and best wishes,
Rich
Richard Friedman,
Columbia University
Here is my ouput from read_distribution.py from RSEQC run from Galaxy
Total Reads 54875279
Total Tags 58170116
Total Assigned Tags 54031503
=====================================================================
Group Total_bases Tag_count Tags/Kb
CDS_Exons 39184887 10217883 260.76
5'UTR_Exons 37214570 10963357 294.60
3'UTR_Exons 58010731 4386101 75.61
Introns 1539911688 6018229 3.91
TSS_up_1kb 24518972 906468 36.97
TSS_up_5kb 110212503 4956374 44.97
TSS_up_10kb 201827137 13004223 64.43
TES_down_1kb 27333233 59583 2.18
TES_down_5kb 118264548 3689226 31.19
TES_down_10kb 210002921 9441710 44.96
My questions:
Where does rRNA fit in this picture?
What about other transcripts other than mRNA such as pseudogenes?
Thanks and best wishes,
Rich
Richard Friedman,
Columbia University