Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • poisson200
    Member
    • Feb 2010
    • 63
    #1

    Tophat; warning

    I have ran Tophat on 50bp paired-end Illumina data and it generates quite a few warnings as below.

    [Thu Mar 3 09:35:27 2011] Indexing splices
    Warning: Encountered reference sequence with only gaps
    Warning: Encountered reference sequence with only gaps
    Warning: Encountered reference sequence with only gaps
    many more lines of this.

    Is this something to be concerned about? I am using HG19 which was indexed using the latest Bowtie-build;

    bowtie-build version 0.12.7
    TopHat v1.2.0

    Thanks for any tips.

    John.
    Tags: None
    Previous template Next

    Latest Articles

    Collapse
    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Gene Copies Once Dismissed as Junk DNA Shape Animal Adaptation and Human Disease by SEQadmin2
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    27 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    11-12-2026, 07:59 AM  
    WashU Medicine Study Verifies Methods for Tracking Genetic Mosaicism by SEQadmin2
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    11-05-2026, 09:25 AM  
    HuBMAP Portal Offers Free Access to Single-Cell Data from Healthy Human Tissues by SEQadmin2
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    		 Last Post kinpict.com
    by kinpict.com
    Today, 06:49 PM  
    Genome Sequencing Reveals Large, Repeat-Rich DNA in Trapdoor Spider Named for Stephen Colbert by SEQadmin2
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    10-28-2026, 09:57 AM  
    View All
    Working...