We just finished analysis on a RNA-SEQ project, however, we found the exonic reads accounts for only 15% of total reads while intergentic reads almost reached 70%. Very confused. Any thoughts here?
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Maybe the sample is contaminated by the genome DNA ? -
We have seen a similar thing with one set of libraries and it was confirmed as carryover DNA contamination.Originally posted by CC_seqanswers View Post
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I suspected DNA contamination too and thank you for confirming the case!
Originally posted by protist View PostWe have seen a similar thing with one set of libraries and it was confirmed as carryover DNA contamination.Comment
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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