We just finished analysis on a RNA-SEQ project, however, we found the exonic reads accounts for only 15% of total reads while intergentic reads almost reached 70%. Very confused. Any thoughts here?
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Maybe the sample is contaminated by the genome DNA ? -
We have seen a similar thing with one set of libraries and it was confirmed as carryover DNA contamination.Originally posted by CC_seqanswers View Post
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I suspected DNA contamination too and thank you for confirming the case!
Originally posted by protist View PostWe have seen a similar thing with one set of libraries and it was confirmed as carryover DNA contamination.Comment
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