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  • richenlaw
    Junior Member
    • Apr 2009
    • 1

    #1

    De novo assembling taking into account base quality

    Hi, everyone!

    I would like to ask that if there is a program taking into account the quality lines of fastq files while doing illumina paired end de nono assembling.

    The most popular illumina paired end assembler, say velvet, doesn't take advantage of the quality scores even inputting a fastq file. This is confirmed by the author on velvet website.

    Any help would be appreciated.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    MIRA can take paired end Illumina data, and uses the quality scores.

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