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  • litc
    Member
    • Oct 2010
    • 24

    What is GO term

    Hello, I'm a newer, I don't know about GO term, what's that mean.

    I have a protein sequence, I blast it to NR and got that info:

    >ref|ZP_05687769.1| acyltransferase 3 [Staphylococcus aureus A9635]
    gb|EEV68775.1| acyltransferase 3 [Staphylococcus aureus A9635]
    Length=603

    Score = 1216 bits (3147), Expect = 0.0, Method: Compositional matrix adjust.
    Identities = 600/603 (99%), Positives = 602/603 (99%), Gaps = 0/603 (0%)

    Query 1 MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSGGFLGVDTFFVISGYLI 60
    MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSGGFLGVDTFFVISGYLI
    Sbjct 1 MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSGGFLGVDTFFVISGYLI 60

    Query 61 TSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFTLIFKPELIIQMKRDAIAA 120
    TSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFTLIFKPELIIQMKRDAIAA
    Sbjct 61 TSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFTLIFKPELIIQMKRDAIAA 120

    Query 121 IFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLLFPLVITFLLHRFKPRNIIQT 180
    IFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLLFPLVITFLLHRFKPRNIIQT
    Sbjct 121 IFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLLFPLVITFLLHRFKPRNIIQT 180

    Query 181 LFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLLGCILAFIWPPFALKKDISKKIV 240
    LFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLLGCILAFIWPPFALKKDISKKIV
    Sbjct 181 LFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLLGCILAFIWPPFALKKDISKKIV 240

    Query 241 VSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYIISFATLFIIAIAVHPSSLFAKFLSM 300
    VSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYIISFATLFIIAIAVHPSSLFAKFLSM
    Sbjct 241 VSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYIISFATLFIIAIAVHPSSLFAKFLSM 300

    Query 301 KPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIPVYVYIIEILLTALMAEISYRFIETPI 360
    KPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIPVYVY+IEILLTALMAEISYRFIETPI
    Sbjct 301 KPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIPVYVYVIEILLTALMAEISYRFIETPI 360

    Query 361 RKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEKKEKKTEFKTT 420
    RKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEKKEKKTEFKTT
    Sbjct 361 RKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEKKEKKTEFKTT 420

    Query 421 KKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPNAQIDGKVGRQLV 480
    KKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPNAQIDGKVGRQLV
    Sbjct 421 KKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPNAQIDGKVGRQLV 480

    Query 481 DATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKADIYLVSIRVPRDYEG 540
    DATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKADIYLVSIRVPRDYEG
    Sbjct 481 DATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKADIYLVSIRVPRDYEG 540

    Query 541 RINKLIYEAAEKRSNVHLVDWYKASAGHPEYFAYDGIHLEYAGSKALTDLIVKTMETHAT 600
    RINKLIYEAAEKRSNVHLVDWYKAS GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHAT
    Sbjct 541 RINKLIYEAAEKRSNVHLVDWYKASEGHPEYFAYDGIHLEYAGSKALTDLIVKTMETHAT 600

    Query 601 NKK 603
    NK+
    Sbjct 601 NKR 603

    Is the go term in the string : acyltransferase 3 [Staphylococcus aureus A9635]
    gb|EEV68775.1| acyltransferase 3 [Staphylococcus aureus A9635],
    if so, what is it?
  • jimmybee
    Senior Member
    • Sep 2010
    • 119

    #2
    GO is gene ontology.

    What you have there is the genbank accession, functional name for the protein followed by the organism and strain number. No GO term

    Comment

    • litc
      Member
      • Oct 2010
      • 24

      #3
      Originally posted by jimmybee View Post
      GO is gene ontology.

      What you have there is the genbank accession, functional name for the protein followed by the organism and strain number. No GO term
      How could I get that protein sequence 's GO term? I really don't know about it.

      Comment

      • jimmybee
        Senior Member
        • Sep 2010
        • 119

        #4
        Originally posted by litc View Post
        How could I get that protein sequence 's GO term? I really don't know about it.
        Have a look at websites like KEGG or just search gene ontology in google. There are quite a few sites where you can put in accessions of gene terms and get out GO terms

        Comment

        • litc
          Member
          • Oct 2010
          • 24

          #5
          Originally posted by jimmybee View Post
          Have a look at websites like KEGG or just search gene ontology in google. There are quite a few sites where you can put in accessions of gene terms and get out GO terms
          Thank you very much for your quick reply, I very very appreciate it. but I'm really a newer, I got to KEGG, but don't know how to use it.

          I'm interested in webs that can convert accession ids to GO terms, I google gene ontology, but found no usable info for me. could you give to some webs that can convert accession ids to GO terms.

          Comment

          • AdamB
            Member
            • Apr 2010
            • 43

            #6
            DAVID is quite useful for GO analysis. Paste in your IDs, and specify what type they are.

            Comment

            • jimmybee
              Senior Member
              • Sep 2010
              • 119

              #7
              Originally posted by litc View Post
              How could I get that protein sequence 's GO term? I really don't know about it.
              You could just use BLAST on KEGG or UniProt databases to find the gene info (which generally has the GO annotations). In the case of uniProt you need the EMBL protein ID

              QuickGO is good for this as well. Here's the GO annotations for your protein:

              Comment

              • litc
                Member
                • Oct 2010
                • 24

                #8
                Originally posted by jimmybee View Post
                You could just use BLAST on KEGG or UniProt databases to find the gene info (which generally has the GO annotations). In the case of uniProt you need the EMBL protein ID

                QuickGO is good for this as well. Here's the GO annotations for your protein:
                http://www.ebi.ac.uk/QuickGO/GProtein?ac=C8MHN2
                Thank you very much, I'll try it and see how far can I get.

                Comment

                • litc
                  Member
                  • Oct 2010
                  • 24

                  #9
                  Originally posted by AdamB View Post
                  DAVID is quite useful for GO analysis. Paste in your IDs, and specify what type they are.
                  Thank you very much, I'm trying it.

                  Comment

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