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  • najoshi
    Member
    • Feb 2010
    • 20

    #1

    samtools colorspace calmd is incredibly slow

    Hi all,

    I'm running samtools version 0.1.14 (and bwa version 0.5.8c) and I am
    trying to do solid colorspace analysis on some single-end reads. I
    indexed the reference using colorspace and I also did the alignment
    and samse using colorspace after converting the solid files using the
    solid2fastq.pl script. Now I am trying to run samtools calmd on the bam files
    and the process is incredibly slow. For example, I have a 1G
    reference and a 2.4G bam file and the process has been running for
    about 3 days now and only about 98M of data have been outputted. The
    data does keep growing, however, so I know that the process is writing
    and not hung, it's just writing at an incredibly slow rate. I am
    running this on an 8 core, 144G ram machine. Am I doing something
    wrong? Has anyone else seen this behavior?

    Here is the calmd command I used:

    samtools calmd -Ar input.bam reference.fa > output.calmd.bam

    Any help would be highly appreciated. Thanks!

    - Nik.
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    For those who didn't see the response on the samtools mailing list, try sorting your file first.

    http://biostar.stackexchange.com/que...ibly-slow/7060
    Last edited by nilshomer; 03-31-2011, 07:37 PM.

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