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  • shiv076
    Junior Member
    • Feb 2022
    • 1

    #1

    Problem using ANNOVAR for Gene-based annotation

    Hello,

    I am trying to find cancer-specific mutations in the sequenced data.

    I am using Samtools and ANNOVAR as a combination.

    I was able to create a VCF file with variants calling using Samtools.

    Now I am trying to annotate using ANNOVAR. But when I run the command

    " table_annovar.pl vcfinput humandb/ -buildver hg38 -out myanno -remove -protocol cosmic -operation f -xref hg38_cosmic_database.txt"

    I am getting the following eroor
    invalid record found in VCF4 file (at least 8 tab-delimited fields expected):

    I am not able to find how to eliminate this error.

    Can someone please help with it?

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