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  • tatresi
    Junior Member
    • Sep 2014
    • 2

    #1

    help finding percentage of insertion present

    I have some paired end reads that were aligned to a reference using an in-house pipeline. There was a big coverage dip in at a certain point on the reference. Looking at the bam alignment in geneious, we could see there was an insertion relative to the reference. After creating a new reference with the insertion included, the coverage looks great.

    Now I need to find out what percentage of the reads have this insertion relative to the reference. It sounds like a pretty simple task, but I'm having trouble figuring it out. Any advice?

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