Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • PFS
    Member
    • Mar 2010
    • 55

    #1

    mapped vs. aligned in TopHat and flagstat

    Sorry if this is a silly question.

    Assuming here mapped ~ aligned.

    My understanding is that TopHat output contains only alignment information. Therefore, not all input reads make it into the output, because not all reads align.

    Can I conclude that because the unmapped reads don't make it in the Tophat output, the flagstat tools will always return 100% of reads mapped on a tophat output?
  • Jon_Keats
    Senior Member
    • Mar 2010
    • 279

    #2
    That is correct

    Comment

    • PFS
      Member
      • Mar 2010
      • 55

      #3
      Thus, considering a tophat output (BAM format), why do I see reads with flags that indicate the reads are unmapped? Is that because those unmapped reads have a mate that is mapped ?

      Comment

      Latest Articles

      Collapse

      • kinpict.com
        Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by kinpict.com
        The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
        Today, 06:37 PM
      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 11-12-2026, 07:59 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      2 responses
      56 views
      0 reactions
      Last Post kinpict.com  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      175 views
      0 reactions
      Last Post SEQadmin2  
      Working...