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  • PFS
    Member
    • Mar 2010
    • 55

    #1

    mapped vs. aligned in TopHat and flagstat

    Sorry if this is a silly question.

    Assuming here mapped ~ aligned.

    My understanding is that TopHat output contains only alignment information. Therefore, not all input reads make it into the output, because not all reads align.

    Can I conclude that because the unmapped reads don't make it in the Tophat output, the flagstat tools will always return 100% of reads mapped on a tophat output?
  • Jon_Keats
    Senior Member
    • Mar 2010
    • 279

    #2
    That is correct

    Comment

    • PFS
      Member
      • Mar 2010
      • 55

      #3
      Thus, considering a tophat output (BAM format), why do I see reads with flags that indicate the reads are unmapped? Is that because those unmapped reads have a mate that is mapped ?

      Comment

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