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  • markestine
    Junior Member
    • Apr 2011
    • 2

    #1

    GSNAP and GFF

    Hello,

    I try to use GSNAP to map RNAseq on human genome.

    I built the gmapdb and I created the file .iit whith the human_GFF:
    #iit_store -G -l ID -o CHR37 HomoCH37.gff3

    When I run GSNAP whith this command :
    # gsnap -d CHR37 -m 6 --nofails -A sam -s CHR37 -N 1 -S 1 -B 4 -t 2
    fastq_seq
    I have this error message :
    "Reading splicesite file
    /usr/local/share/gmapdb/CHR37/CHR37.maps/CHR37...Splicesite file
    CHR37.iit does not have tag 'donor'."

    I don't understand why I have this message?
    I would like used the GFF when I map the RNASeq reads on the human
    genome to take into account the splicing.

    Can you help me resolve this problem?

    Thanks in advance,
    markestine

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