Hi all,
I'm new to RNA seq and I'm working with TopHat - Cufflinks - Cuffcompare - Cuffdiff pipeline.
I use Cufflinks without annotation and then Cuffcompare for two samples with annotation. I put the results of Cuffcompare (transcripts.combined.gtf) to Cuffdiff as input. Is this the right way?
I have results from cuffdiff for differential expression testing. As a result for DE testing it provides the following measure: ln(FPKMx/FPKVy) for ratio for the gene between two samples X and Y. If for gene A in sample X FPKMx = 2 and for the sample Y FPKMy=0, then the ratio is like 1,79 E306, so enormously big
How do you deal with it?
Any ideas are very welcome!
I'm new to RNA seq and I'm working with TopHat - Cufflinks - Cuffcompare - Cuffdiff pipeline.
I use Cufflinks without annotation and then Cuffcompare for two samples with annotation. I put the results of Cuffcompare (transcripts.combined.gtf) to Cuffdiff as input. Is this the right way?
I have results from cuffdiff for differential expression testing. As a result for DE testing it provides the following measure: ln(FPKMx/FPKVy) for ratio for the gene between two samples X and Y. If for gene A in sample X FPKMx = 2 and for the sample Y FPKMy=0, then the ratio is like 1,79 E306, so enormously big
How do you deal with it?
Any ideas are very welcome!
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