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  • adrian
    Member
    • Oct 2009
    • 90

    #1

    TopHat Junctions visualization help

    Hi:

    I need some help in interpreting Junctions.bed in a browser.

    I have 4 different junction.bed files from 4 different samples. I uploaded them in IGV (Broad) and I see the transcripts. File attached.

    (link: http://img864.imageshack.us/f/nupr1.jpg/ )

    For instance in NUPR1 gene, all 4 junction files have trascripts that overlap CCDC101 and IL27 genes.

    1. Why are transcripts starting in CCDC101 gene and ending in IL27. Is this because of novel TSS for CCDC101 and novel TES.

    2. What is the meaning of <> and << between exons?
    it is my understanding that < or > denotes direction + or - ve of Transcription. then what does <> and << or >> denote?

    Thanks for your help.

    Adrian

    PS: I tried uploading smaller image file, however the best compression I could get to 30Kb.
    thus I am uploading file to this free image hosting site I found. Apologies if this is not allowed.

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