Separate forward and reverse coverage in Artemis

[nivea]

Hi all,

Do you know how to demonstrate the reverse strand in the <0 region when you input *.BAM file including all the mapped reads in both forward and reverse strand?

[nilshomer]

This question is not making sense to me. Could you clarify?

[nivea]

This question is not making sense to me. Could you clarify?

Hi, sorry for not clear enough.
After you read a BAM file(a mapped RNA-seq reads file) using Atermis, you'll see the expressed coding regions covered by many overlaped reads. However, the mapped reads from both forward strand and reverse strand are piled up in the above zero region. I'm considering a way to differentiate these reads, such that the forward strand reads can be piled up in the above zero region while the reads from the reverse strand can be piled up in below zero region...

[moodymj]

I'm having the same issue. Has anyone found a solution?

[maubp]

Have you explored the right click menu? Artemis/BamView has several ways of displaying BAM files and I think one of them is strand specific.