Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • arvid
    Senior Member
    • Jul 2011
    • 156

    #16
    Originally posted by pardonliang View Post
    ... I have used susceptible、resistance 、susceptible and resistance assebled transcriptomes as reference to compare the number of paired-maping reads、singled-mapping reads、up expressed transcripts.The result of stored in Attach Files.
    According to your question(the software and statistics used, the amount and type of replicates, the biological system you are working on, and the way the samples were collected).I haven't used statistics、replicateds yet...
    Alright, so don't expect much from your experiment, since you have different strains and no biological replicates... You specify "RPKM>2" in your table, was this log-transformed RPKM values or just raw RPKM? In any case, I really wouldn't recommended using an arbitrary expression-level focused cutoff, and I guess you can't evaluate those thousands of genes anyway. You might want to feed your counts through edgeR or DESeq in R/Bioconductor instead and use the options for experiments without biological replicates (there are good instructions in their respective manuals, or just search SEQanswers). You will not see a lot of differentially expressed transcripts then, but hopefully you can reduce the number of falsely called differentially expressed transcripts. You should afterwards check that the observed differential expression is not due to lower mapping due to polymorphisms in one of the samples, however.
    You could try IsoEM or RSEM as well, though I'm not sure how sensitive their respective EM algorithms are to polymorphisms in the underlying mappings. We used RSEM in a similar case to yours (I wasn't involved until a late stage), and were able to identify some qualitatively DE genes (confirmed by RT-qPCR). I can't recommend it again though - replicates are really necessary to get information of high quality out of such an experiment.

    Comment

    • shoegame2001
      Member
      • Dec 2010
      • 21

      #17
      I apologize if this question has been addressed elsewhere--I did a little bit of looking but could not find a consensus:

      When combining assemblies of different kmer lengths (in my case using Trinity), what is the best method for eliminating redundancy in the resulting combined assembly to make the most comprehensive reference possible? I have tried using cdhit-est, TGICL, and CAP3, but am still observing redundancy when I blast my assembly to itself. For those who have had success with these tools, what options were you using?

      Thanks!

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
        by SEQadmin2



        CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

        Despite this, “CRISPR helped turn genome editing from a specialized technique into
        ...
        07-31-2026, 11:01 AM
      • SEQadmin2
        Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
        by SEQadmin2


        Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

        The systematic characterization of the human proteome has
        ...
        07-20-2026, 11:48 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 08-06-2026, 07:41 AM
      0 responses
      17 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 08-03-2026, 10:13 AM
      0 responses
      33 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-31-2026, 02:55 AM
      0 responses
      42 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-24-2026, 12:17 PM
      0 responses
      26 views
      0 reactions
      Last Post SEQadmin2  
      Working...