Hi,I have a question ,I used illumina single-end 32mer read to ran tophat on galaxy,and then use hit.bam ran cufflink but fail(error).Does anyone know can cufflink run sinle-end reads? Thanks!
-
-
Maybe the cufflinks can't read the bam files. I remember that cuffllinks need sam files as its input.Change bam file to sam file with samtool and have another try. -
Originally posted by feixue1039 View Post
But in my other case,I ran paired-end read in tophat,and also use hit.bam to run cufflink is OK.
Therefore,I guest cufflink on galaxy's parameters, not apply to single-end readsComment
-
THanks,Everyone,I know the ansewr.Your hit.bam need to edit source of database:hg19.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment