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  • LucasVS
    Junior Member
    • May 2011
    • 1

    #1

    Multiple Alignment software for huge amount of peptide sequences/cysteine framework

    ***edit***
    Last edited by LucasVS; 04-15-2012, 10:25 PM.
  • colindaven
    Senior Member
    • Oct 2008
    • 417

    #2
    I wouldn't align with Excel .... scripts are very slow in it, and I doubt you'll find a bioinformatician willing to touch it!

    Why not try muscle, thats a nice quick bit of software. Perhaps muscle can also align cysteines only, with the gap characters (-) you put in. Otherwise maybe subsitute all other amino acids with a fairly neutral amino acid (perhaps Alanine). You can then see if the alignment is mainly determined by the cysteines.

    Comment

    • me_myself_andI
      Member
      • Nov 2010
      • 30

      #3
      Hello,

      Excel for storing sequence data and/or VBA for scripting is a very bad idea in this context.

      Try the following:
      Convert your sequences to Fasta format (plain text) and use either Muscle (muscle -maxiters 1 -in your-fasta-file) or Mafft (mafft -auto your-fasta-file)

      Andreas

      Comment

      • *#1*
        Junior Member
        • Jul 2010
        • 8

        #4
        I also suggest Muscle http://www.drive5.com/muscle/

        Comment

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