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Yes, i mean "mapping to a reference sequence" and i want to known some other programs designed for alignment of solid or 454 reads. -
If by "aligning" you mean "mapping to a reference sequence" then I would say that the company's offered software will be one of the best options. This is 'corona lite' for the Solid (works in color space!) and Newbler for 454.Originally posted by baohua100 View Post
For cross-platform mapping and/or assembly (i.e., mixing up 454 and SOLiD reads and expecting to obtain contigs from them) then neither of the above will work very well.Leave a comment:
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Alignment of ABI solid reads and 454 reads
Which programs suits aligning ABI solid reads most?
Which programs suits aligning 454 reads most?
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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