Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • coldturkey
    Member
    • Nov 2008
    • 51

    #1

    sff_extract paired end extraction error

    I have been trying to run sff_extract using the -l flag to
    extract the paired end read from my sff file but I receive
    the following error.

    brian@Gaff[Pairedend_extract] sff_extract -o testrun -l
    /home/brian/Neo_home/test/Pairedend_extract/LINKER/Linker.fasta
    /home/brian/Neo_home/test/FE4758201.sff
    [ 1:21PM]
    Working on '/home/brian/Neo_home/test/FE4758201.sff':
    Creating temporary sequences from reads in
    '/home/brian/Neo_home/test/FE4758201.sff' ... done.
    Searching linker sequences with SSAHA2 (this may take a
    while) ... Input parameter error for "-solexa"


    An error occured during the SSAHA2 execution, aborting.

    I am unsure what exactly is causing the error as SSAHA2
    works fine outside of sff_extract and sff_extract works perfectly
    when not using the -l flag

    regards

    Brian
  • BaCh
    Member
    • May 2008
    • 81

    #2
    Wrong version of ssaha, upgrade to one that knows the "-solexa" flag :-)

    B.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Working...