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  • aleferna
    Senior Member
    • Sep 2009
    • 121

    #1

    Exome Affy Chip Analysis

    Hi, I'm looking for the best algorithm to normalize probe levels and detect splicing variants. I've recently tried RMA but when I summarize by gene it misses a lot of genes. What is the best open source tool to evaluate gene expression levels per gene and to generate splicing variant estimates?

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    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
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  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

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