Is there any application can convert gff3 file and related fasta files into ace file or sam file? I dont want to reinvent the wheel.
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I don't understand what you want to do - going from GFF3 to ACE or SAM makes no sense.
You store annotation in GFF3 files (e.g. there is a gene at this location), but neither ACE nor SAM hold that kind of annotation (they are assembly/mapping files, e.g. this read mapped to this contig at this location).
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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