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  • pinki999
    Member
    • Oct 2010
    • 37

    #1

    Problem using HTSeq

    Hi,

    I am currently using HTSeq to get raw counts. HTSeq has been installed on our linux cluster and the administrators suggested to use export PATH=... in order to use it. However, I am getting following error:

    Traceback (most recent call last):
    File "/opt/HTSeq/bin/htseq-count", line 3, in ?
    import HTSeq.scripts.count
    ImportError: No module named HTSeq.scripts.count

    I am not able to figure out the reason. Anyone of you faced the same problem and found solution?

    Please share your suggestions.


    Thank you,

    Pinki
  • poojitharao
    Junior Member
    • May 2010
    • 2

    #2
    I have the same issue! Could someone help with this?
    Did you figure it out already pinky?

    Comment

    • Simon Anders
      Senior Member
      • Feb 2010
      • 995

      #3
      HTSeq has been installed incorrectly. The PATH setting allows your shell to find the htseq-count script in its non-standard location in /opt/HTSeq/bin/, but this script is just a three-line file that tell Python to load HTSeq and the 'count' submodule. As HTSeq has not been installed into the standard Python package path, you also have to set the PYTHONPATH variable. I believe that the distutils install function that I used displays a warning about this whenever it is asked to install to a non-standard location.

      It might be easier if you installed HTSeq yourself with 'python setup.py install --user', as suggested in the installation instructions.

      Comment

      • Pseudoknot
        Junior Member
        • May 2012
        • 4

        #4
        Hi Simon,

        I have the same problem as pinky, I installed setuptools and then installed HTSeq using 'python setup.py install --user' as suggested. Still, htseq-count is not added to my path. I did "import HTSeq" on a python shell and I get no errors. Do you have any suggestions? I'm running python 2.7.3.

        Comment

        • Pseudoknot
          Junior Member
          • May 2012
          • 4

          #5
          Issue solved, the script was added to ./local/bin instead of /bin. Thanks.

          Comment

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