Hy, we made a 454 run of a miRNA library of a non sequenced plant. What pipeline you would suggest to predict novel miRNA considering that we have not any reference ?
Thank you very much
Thank you very much
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You can use the genome of a very related plant.
Otherwise I don't know how to do such prediction without a reference genome.. -
There are some tools which predict the precursor mirna structure from the sequence. If im not mistaken they used HMM to do that, but I cannot remember the name of the tool.Comment
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Thank you very much. I am in diffuculty about this, i'll try different ways.Comment
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