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  • naumenko.sa
    Junior Member
    • May 2011
    • 5

    #1

    Illumina reads with dots

    Some reads from Illumina (qseq file) have dots at the beginning and in the middle of the read.
    ......CTTTTTAAGCCACAGCATGCTCAGGAAGTTACGATATTGCAAAGGATTTACTGTGACGTGGGTGAAATGTTACTCTCCTCCGATATTGCTGTAACCGATCA..TCAAACCCT.TGGCGA.TT.AGAGTCAAAGCCCATTACCAGCTGCGCCTAC
    What does it mean?
  • kmcarr
    Senior Member
    • May 2008
    • 1181

    #2
    It means that the base caller was unable to determine the base at that position. When the reads are converted to FASTQ format the dots will be replaced with N's.

    Comment

    • naumenko.sa
      Junior Member
      • May 2011
      • 5

      #3
      Thank you, kmcarr.

      Comment

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