Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • captainobvious
    Junior Member
    • Feb 2009
    • 2
    #1

    Manually correct heterozygous indels

    Hi there!

    Does anyone know how to deal with heterozygous indels manually? I don't know how to deal with cases where sequence in one direction shows double peaks while the sequence in the opposite direction shows NO peaks at those sites. I'm thinking that it would be right to ignore those peaks. I could be wrong. I'm just not sure...
    Tags: None
    • swbarnes2
      Senior Member
      • May 2008
      • 910
      #2
      Make two reference sequences, one with the indel, one without. The reads that best align to the indel will align to that sequence, the ones without will best align to the other.

      That'll help you figure out of the reads are real.

        Comment

        • captainobvious
          Junior Member
          • Feb 2009
          • 2
          #3
          Ok, I thought that would be the way to go. Just wanted a second opinion. Thanks!!!

            Comment

            Previous template Next

            Latest Articles

            Collapse
            • SEQadmin2
              Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
              by SEQadmin2



              Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

              Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating              ...
              10-14-2026, 09:55 AM
            • SEQadmin2
              New Genomics Technologies Take Aim at Long-Standing Limits
              by SEQadmin2


              Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

              We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing              ...
              09-28-2026, 10:25 AM
            View All

            ad_right_rmr

            Collapse

            News

            Collapse
            Topics Statistics Last Post
            Exeter-Led Study Maps What an Average Human Genome Looks Like by SEQadmin2
            Started by SEQadmin2, Yesterday, 11:13 AM
            0 responses
            16 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            Yesterday, 11:13 AM  
            Long-Read Sequencing Uncovers Cancer Mutations Conventional Testing Missed by SEQadmin2
            Started by SEQadmin2, Yesterday, 10:48 AM
            0 responses
            17 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            Yesterday, 10:48 AM  
            Mayo Clinic-Led Study Detects DNA Mutations Across Noncancerous Tissues by SEQadmin2
            Started by SEQadmin2, 10-15-2026, 10:52 AM
            0 responses
            35 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-15-2026, 10:52 AM  
            Study Finds Chromatin Motion Holds Steady Until Cells Face Stress by SEQadmin2
            Started by SEQadmin2, 10-14-2026, 05:39 AM
            0 responses
            42 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-14-2026, 05:39 AM  
            View All
            Working...