Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • srividya
    Junior Member
    • Sep 2010
    • 6

    #1

    Samtools pileup Consensus with 0.1.12a version

    Hello,

    I am using samtools 0.1.12a version for pileup consensus variant calling (-vc). I have aligned the reads with Soap Aligner and I am trying to call the consensus SNPs. Though, the pileup(only -f option) for the bam file is getting generated, I am unable to generate the consensus (-vc option) with it. I checked the CIGAR and also the sam file. They are pretty much looking normal. Can anyone tell what might be the problem?

    Thanks,
    Srividya
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    Post a small set of reads that give this problem. Also, consider moving to the latest version of samtools, which uses "mpileup" instead of "pileup".

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...