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  • john@wurbio
    Junior Member
    • May 2011
    • 2
    #1

    reverse run -due to machine failure- only 45bp

    Hi all,

    I have a rather unusual question for which I get conflicting answers up to know. Hope anyone can be of help.
    We have made a RRLibrary of 100-200bp fragments for GA2 sequebcing. The forward run is 100bp and the reverse run -due to machine failure- only 45bp. How does this influence assembly compared to 100bp from both runs? Do we n
    eed to adjust settings in a short read assembler?

    Thanks!
    Tags: None
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