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  • Sylphide
    Member
    • Feb 2011
    • 11

    #1

    SAM/BAM how to retrieve all mapped reads ?

    Hello,
    I obtained a BAM file from BWA and would like to keep only mapped reads. Samtools view -q 1 removes reads with mapQ 0 and I would like to conserve them.
    Does anyone have a solution ?
    Many thanks
    Last edited by Sylphide; 05-30-2011, 12:39 PM.
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    samtools view -F 4

    See: http://picard.sourceforge.net/explain-flags.html

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    • prisnirath
      Member
      • May 2011
      • 19

      #3
      Hi...
      I used BWA to get a sam file and used SamTools to call the variants in my reads, got huge no. of variants.
      Whe I viewed my sam file using tablet app., I got hold of datasets showing variants with read depth 1.
      how do I manipulate the read depth and set its min. value to be around 50 or so to call in genuine SNPs?

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