Problem with visualizing bigwig files... Help!

[anagari]

Hi all,

I having big-time trouble in viewing my bigwig files in UCSC genome browser. I am trying to upload them in sftp (ssh ftp).

Using:
track type=bigBed name="bigBed Example One" description="A bigBed file" bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigBedExample.bb

I dont have ftp server all I have is ssh ftp server account. I uploaded the bigwig file in the server and got the url but the url doesn't seem to work.

I tried giving the server username and password along in url but, the file is not opening in the url instead, it is downloading from the server...

Is there a way to visualize bigwig files using ssh ftp server? or is there a simple way to generate a secure url to upload in ucsc genome browser!!

Thanks in advance!

[anagari]

The error am getting is
Error http://server/filename.bw is not a big wig file

I followed the steps in http://genome.ucsc.edu/goldenPath/help/bigWig.html

but, it still gives this error which is driving me nuts!!

[Richard Finney]

Your example appears to be BigBed, not BigWig.

Can you access the URL by using wget ? example: "wget http://server/filename.bw " ? wget is available on Windows via cygwin and on Linux.
... OR ...
Can you access your file via entering the URL on your browser ?

[anagari]

Yes, the file is accessible through the URL. I used the standard wigToBigWig conversion steps listed in http://genome.ucsc.edu/goldenPath/help/bigWig.html

and tried to upload the file onto the server.. it says that the file is not bigwig...

[anagari]

The problem is solved!