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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

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  • seqadmin
    Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
    by seqadmin



    Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
    03-21-2023, 01:49 PM
  • seqadmin
    Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
    by seqadmin




    Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
    03-10-2023, 05:31 AM
  • seqadmin
    Expert Advice on Automating Your Library Preparations
    by seqadmin



    Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....
    02-21-2023, 02:14 PM

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