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  • Silvia Weidner
    Junior Member
    • Jun 2012
    • 5
    #16
    Problems in shearing

    Hi erverybody,
    i am new in this field. We have a pgm and i started the gDNA shearing.
    I have big problems to get the right size.
    Can you give me some tips how you optimize your shearing? On which aperatur do you check your sizes? Is it mormal to see only smear?

    Thanks a lot
    Greetings from Munich

    Silvia

      Comment

      • rnaeye
        Member
        • May 2011
        • 80
        #17
        Why do not you try enzymatic shearing instead. You can use plate format:
        NEBNext® dsDNA Fragmentase®
        Page Not Found | NEB
        http://www.neb.com/nebecomm/products/productm0348.asp
        This is the default text for fallback 404 continue text

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